Journal: Cell Death & Disease

Article Title: Prosapogenin A induces GSDME-dependent pyroptosis of anaplastic thyroid cancer through vacuolar ATPase activation-mediated lysosomal over-acidification

doi: 10.1038/s41419-024-06985-z

Figure Lengend Snippet: A ATC cells (8505C, KHM-5M, C643, BHT-101, HTh-7) were treated with 0, 2, 3, 4, 5, 6, 7, 8, and 9 μM PA for 24 h. Relative cell viability was assessed by CCK8. B The clone formation images of 8505C and KHM-5M after PA treated at 0, 1, 2, 3 μM for 2 weeks, and quantified by the ImageJ software C 8505C and KHM-5M cells death was detected by Calcein-AM/PI Double Stain Kit, and morphologies were photographed by an Invitrogen EVOS-M 7000 microscope, after PA treated for 24 h at indicated concentrations, (scale bar: 200 μm). D 8505C and KHM-5M cell death was measured by flow cytometry after PA treatment for 24 h at 0, 4, 5, 6 μM. E PARP, cleaved-PARP and GAPDH protein levels in PA (0, 4, 5, 6 μM), ATL (20 μM) and STS (0.5 μM) treated for 24 h ATC cells (8505C and KHM-5M) were detected by western blot. Data are shown as mean ± SD for n = 3 (biological replicates). * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: B The clone formation images of 8505C and KHM-5M after PA treated at 0, 1, 2, 3 μM for 2 weeks, and quantified by the ImageJ software C 8505C and KHM-5M cells death was detected by Calcein-AM/PI Double Stain Kit, and morphologies were photographed by an Invitrogen EVOS-M 7000 microscope, after PA treated for 24 h at indicated concentrations, (scale bar: 200 μm).

Techniques: Software, Staining, Microscopy, Flow Cytometry, Western Blot